Image Analysis of HER2 Immunohistochemical Staining of Surgical Breast Cancer Specimens

Cytoplasmic Her2/neu immunohistochemical staining in breast cancer; From a molecular point of view

Optimization of Real Time PCR for Precise Measurement of HER2 Overexpression in Breast Cancer Specimens

Background: Breast cancer is one of the most prevalent malignancies among women in various countries. HER2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. HER2 gene encodes an 185kDa transmembrane glycoprotein with 1255 amino acids. This active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. These...

Analysis of HER2 gene amplification using Differential PCR in breast cancer patients of Isfahan Province

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...

HER2 amplification status in breast cancer: a comparison between immunohistochemical staining and fluorescence in situ hybridisation using manual and automated quantitative image analysis scoring techniques.

AIMS To compare the results of breast cancer sections with HercepTesttrade mark immunohistochemistry (IHC) scores ranging from 0 to 3+ with fluorescence in situ hybridisation (FISH) for HER2 amplification. The HER2 digital scoring application of the Micrometastasis Detection System (MDS) was used, together with manual scoring of FISH and HercepTest, to determine whether this system provides an ...

optimization of real time pcr for precise measurement of her2 overexpression in breast cancer specimens

background: breast cancer is one of the most prevalent malignancies among women in various countries. her2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. her2 gene encodes an 185kda transmembrane glycoprotein with 1255 amino acids. this active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. these...